Ghost Traps are used to remove impurities from mobile phases. In addition to the regular Ghost Trap DS*, a DS-HP model is now available, which is designed to use with UHPLC systems up to 100 MPa. The Ghost Trap DS-HP is able to remove impurities that get mixed with the mobile phase in preparation for it or that originate from the system, even if the impurities are contained in an organic solvent.
When the Ghost Trap DS is installed between the gradient mixer and autosampler in reversed phase gradient analysis, it can trap impurities from the piping and gradient mixer in addition to those from the mobile phase.
* DS: Abbreviation of Daiichi Sankyo (D) and Shimadzu (S)
The example below shows that it can effectively trap impurities in mobile phase, when the Ghost Trap DS is installed just downstream of the gradient mixer as displayed in the figure below.
Installation of the Ghost Trap™
When the Ghost Trap DS/DS-HP is not used, quantitation of an impurity peak is difficult due to the effects of ghost peaks. The Ghost Trap DS/DS-HP effectively eliminates the ghost peaks to allow quantitation of the target component.
| Column: |
ODS column |
| Mobile phase A: |
25 mmol/L Phosphate (Potassium) buffer solution pH 4.0 /Acetonitrile = 9/1 |
| Mobile phase B: |
Water/Acetonitrile = 1/9 |
| Flow rate: |
0.65 mL/min |
| Oven: |
45 ºC |
| Detection: |
UV 210 nm |
Example of removing ghost peaks by Ghost Trap DS (The data is provided by Daiichi Sankyo Co., Ltd.)
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